Yaqoub Ashhab

Yaqoub Ashhab

Associate Professor of molecular biology and bioinformatics

Publications (peer reviewed journals)

  1. Salah-Alddin Al-Jubeh, Asmaa Tamimi, Yaqoub Ashhab. Design and Optimization of a Genotyping Assay for the CYP2C19*2 Pharmacogentic Marker Based on the SuperSelective Primer Method. (in preparation).
  2. Haneen Tartory, Hashem Tamimi, Yaqoub Ashhab. Impact of pre-Clustering on the Classification of Heterogeneous Protein Data. 2022-11:3. Network Modeling Analysis in Health Informatics and Bioinformatics.
  3. Bessan Aljanazreh, Khaled Alzatari, Asmaa Tamimi, Mohammad H. Alsaafeen, Waheed Hassouneh and Yaqoub Ashhab. Brucellosis Reemergence after a Decade of Quiescence, Palestine, 2015-2017. 2021 Jul 31 Transboundary and Emerging Diseases.
  4. Yasmin Hisham, Yaqoub Ashhab, Sang-Hyun Hwang, Dong-Eun Kim. Identification of highly conserved SARS-CoV-2 antigenic epitopes with wide coverage using reverse vaccinology approach. Viruses. 2021, 13, 787.
  5. Ilaria Zanella, Enrico König, Michele Tomasi, Assunta Gagliardi, Samine J. Isaac, Martina Grigolato, Luca Frattini Francesca Zerbini, Laura Fantappiè, Carmela Irene, Elena Caproni, Riccardo Corbellari, Silvia Valensin, Luca Bini, Yaqoub Ashhab, Alberto Grandi, Guido Grandi (2020) Proteome-minimized Outer Membrane Vesicles from Escherichia coli as a vaccine platform for infectious diseases and cancer. J Extracell Vesicles. 2021 Feb;10(4).
  6. Diala Shatleh-Rantisi, Asmaa Tamimi, Ashhab Y. Improving Sensitivity of Single Tube-nested PCR to Detect Fastidious Microorganisms. Heliyon. 2020 Jan 27;6(1):e03246. doi: 10.1016/j.
  7. R Sultan, H Tamimi, Ashhab Y. Improving Classification Performance Using Genetic Programming to Evolve String Kernels. The International Arab Journal of Information Technology (2019) 16 (3), 454-459.
  8. Yasmin Hisham and Yaqoub Ashhab. Identification of cross protective potential antigens against pathogenic Brucella spp. through combining pan genome analysis with reverse vaccinolog. Journal of Immunology Research. Volume 2018 (2018).
  9. Tamimi A., Ashhab Y., and H Tamimi. Accelerating Information Retrieval from Profile Hidden Markov   Model Databases.  PLOS One Nov 22;11(11):e0166358. doi: 10.1371/journal.pone.0166358.
  10. Noh-Issa M. and Ashhab Y. Identification of Brucella melitensis Rev.1 vaccine-strain genetic markers: Towards understanding the molecular mechanism behind virulence attenuation.  Vaccine 2016 Sep 22;34(41):4884-91.
  11. Qabajah M., Awwad E., and Ashhab Y. Molecular characterization of Escherichia coli from dead broiler chickens with signs of colibacillosis and ready-to-market chicken meat in the West-Bank. British Poult Sci. 2014 Sep 3:1-10
  12. B. Nesta, M. Valeri, A. Spagnuolo, R. Rosini, M. Mora, P. Donato, C.J. Alteri, M. Del-Vecchio, S. Buccato, A. Pezzicoli, I. Bertoldi, L. Buzzigoli. G. Tuscano, M. Falduto, V. Rippa, Ashhab Y., G. Bensi, M.R.Fontana, K. L. Seib, H. L.T. Mobley, M. Pizza, M. Soriani, and L. Serino. "SslE elicits functional antibodies that impair in vitro mucinase activity and in vivo colonization by both intestinal and extraintestinal Escherichia coli strains".  PLOS Pathogens 2014 May 8;10(5):e1004124.
  13. Ayyash M., Tamimi H., Ashhab Y. Developing a Powerful In-Silico Tool for the Discovery of Novel   Caspase-3 Substrates: A Preliminary Screening of the Human Proteome.  BMC Bioinformatics 2012, 13:14.
  14. Czerninski R, Krichevsky S, Ashhab Y, Gazit D, Patel V, Ben-Yehuda D. Promoter hypermethylation of mismatch repair genes, hMLH1 and hMSH2 in oral squamous cell carcinoma.  Oral Dis. 2009 Apr;15(3):206-13.
  15. Nachmias B, Mizrahi S, Elmalech M, Lazar I, Ashhab Y, Gazit R, Markel G, Ben-Yehuda D, Mandelboim O. Manipulation of NK cytotoxicity by the IAP family member Livin. Eur J Immunol. 2007, 37(12):3467-76.
  16. Nachmias B, Lazar I, Elmalech M, Abed-El-Rahaman I, Asshab Y, Mandelboim O, Perlman R, Ben-Yehuda D. Subcellular localization determines the delicate balance between the anti- and pro-apoptotic activity of Livin.  Apoptosis. 2007, 12(7):1129-42.
  17. Ferrer-Francesch X, Caro P, Alcalde L, Armengol MP, Ashhab Y, Lucas-Martin A, Martinez-Caceres EM, Juan M, Pujol- Borrell R. One-tube-PCR technique for CCL2, CCL3, CCL4 and CCL5 applied to fine needle aspiration biopsies shows different profiles in autoimmune and non-autoimmune thyroid disorders. J Endocrinol Invest. 2006, 29(4):342-349.
  18. Colobran R, Adreani P, Ashhab Y, Llano A, Este J, Dominguez O, Pujol-Borrell R, Juan M. "Multiple products derived from two CCl4 loci: high incidence of a new polymorphism in HIV+ patients".  Journal of Immunology. 2005, 174:5655-5664.
  19. Lossos A, Ashhab Y, Sverdlin E, Amir G, Ben-Yehuda D, Siegal T. "Late-delayed cerebral involvement in systemic non- Hodgkin's lymphoma: a second primary tumor or a tardy recurrence?". Cancer.  2004, 101(8):1843-9.
  20. Nachmias B, Ashhab Y, and Ben-Yehuda D. “The Anti Apoptosis Protein Family (IAPs)- An Emerging Therapeutic Target in Cancer”.  Seminars in Cancer Biology. 2004, 14: 231-243.
  21. Nachmias B, Ashhab Y, Bucholtz V, Drize O, Kadouri L, Lotem M, Peretz T, Mandelboim O, and Ben-Yehuda D. “Caspase-Mediated Cleavage Converts Livin from an Anti-apoptotic to a Pro-apoptotic Factor: Implications for Drug- resistant Melanoma”.  Cancer Research. 2003, 63(19):6340-9.
  22. Shteper PJ, Zacharia E, Ashhab Y, Peretz T, Vlodavsky I, and Ben-Yehuda D. “Role of promoter methylation in regulation of the mammalian heparanase gene”.  Oncogene 2003, 22(49):7737-49.
  23. Domínguez O, Sabater L, Ashhab Y, Belloso E, and Pujol-Borrell R. “AU-Differential Display (AU-DD), Reproducibility of a Differential mRNA Display Targeted to AU Motifs”. Methods Mol Biol. 2003; 226:22536.
  24. Sabater L, Ashhab Y, Caro P, Kolkowski EC, Pujol-Borrell R, Dominguez O. “Identification of a KRAB- containing zinc finger protein, ZNF304, by AU-motif-directed display method and initial characterization in lymphocyte activation”.  Biochem Biophys Res Commun. 2002; 293(3):1066-72.
  25. Ashhab Y, Alian A, Polliack A, Panet A, Yehuda DB.Two splicing variants of  a new inhibitor of apoptosis gene with different biological properties and tissue distribution pattern.  FEBS Lett. 2001 Apr 20; 495(1-2):56-60.
  26. Ashhab Y, Dominguez O, Sospedra M, Roura-Mir C, Lucas-Martin A, Pujol-Borrell R. A one-tube polymerase chain reaction protocol demonstrates CC chemokine overexpression in Graves' disease glands. J Clin Endocrinol Metab 1999;84(8):2873-82.
  27. Dominguez O, Ashhab Y, Sabater L, Belloso E, Caro P, Pujol-Borrell R. Cloning of ARE-containing genes by AU-motif- directed display.  Genomics 1998 Dec 1;54(2):278-86.
  28. Sospedra M., Tolosa E, Armengol P, Ashhab Y, Urlinger S, Lucas-Martin A, Foz-Sala M, Jaraquemada D, and Pujol- Borrell R, “Hyperexpression of transporter in antigen processing-1 (TAP-1) in thyroid glands affected by autoimmunity: a contributory factor to the breach of tolerance to thyroid antigen?”  Clin Exp Immunol 1997; 109: 98-106.

Book Chapters

 

1.  Ashhab Y. Bioinformatics and Genomics Sciences in the Middle East; Opportunities and Challenges. New life Sciences; Future Prospects. Biovision 2010. Bibliotheca Alexandrina.

2.  Sasson S, Ashhab Y, Melloul D, Cerasi E. “Autoregulation of glucose transporter: effects of glucose on glucose transporter expression and cellular location in muscle” New Concepts in the Pathogenesis of NIDDM, p113-127, C.G. Ostenson et al., Plenum Press, New York , 1993.

Patents

   Livin-derived peptides, compositions and uses thereof. Accession number at (http://www.ebi.ac.uk): WO2004106371

Program code

·    Berat Kurar, Sami Abu Sneineh, Yaqoub Ashhab.  2016. Protein Descriptors': R package to Generate Various Protein Descriptors for Machine Learning Algorithms. A package for “R” project. https://cran.r- project.org/web/packages/ProteinDescriptors/index.html